Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8199
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dc.contributor.authorFerreira, Lino-
dc.contributor.authorRafael, Ana-
dc.contributor.authorLamghari, Meriem-
dc.contributor.authorBarbosa, Mario A.-
dc.contributor.authorGil, Maria H.-
dc.contributor.authorCabrita, António M. S.-
dc.contributor.authorDordick, Jonathan S.-
dc.date.accessioned2009-02-09T12:16:20Z-
dc.date.available2009-02-09T12:16:20Z-
dc.date.issued2004en_US
dc.identifier.citationJournal of Biomedical Materials Research Part A. 68A:3 (2004) 584-596en_US
dc.identifier.urihttp://hdl.handle.net/10316/8199-
dc.description.abstractThe biocompatibility of chemoenzymatically generated dextran-acrylate hydrogels has been evaluated in vitro, using human foreskin fibroblasts, and in vivo, by subcutaneous and intramuscular implantation in Wistar rats for up to 40 days. In vitro tests show that hydrogel extracts only minimally reduced (<10%) the mitochondrial metabolic activity of fibroblasts. Direct contact of the hydrogels with cells induced a cellular proliferation inhibition index (CPII) of 50-80%, compared with a control, whereas through indirect contact, the CPII values were <16%, suggesting that the high CPII values achieved in the direct assay test were likely due to mechanical stress or limitations in oxygen diffusion. Hence, the hydrogels were noncytotoxic. Moreover, cell-material interaction studies show that these hydrogels were nonadhesive. Finally, histologic evaluation of tissue response to subcutaneous and intramuscular implants showed acceptable levels of biocompatibility, as characterized by a normal cellular response and the absence of necrosis of the surrounding tissues of the implant. In the first 10 days, the foreign-body reaction in the intramuscular implantation was more severe than in subcutaneous implantation, becoming identical after 30 days. In both cases, dextran hydrogels did not show signs of degradation 6 weeks postimplantation and were surrounded by a thin fibrous capsule and some macrophages and giant cells. This response is typical with a number of nondegradable biocompatible materials. These results indicate that dextran hydrogels are biocompatible, and may have suitable applications as implantable long-term peptide/protein delivery systems or scaffolds for tissue engineering. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 68A: 584-596, 2004en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleBiocompatibility of chemoenzymatically derived dextran-acrylate hydrogelsen_US
dc.typearticleen_US
dc.identifier.doi10.1002/jbm.a.20102en_US
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.deptFaculty of Medicine-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0001-8985-9302-
crisitem.author.orcid0000-0001-5165-5849-
Appears in Collections:FCTUC Eng.Química - Artigos em Revistas Internacionais
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