Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8147
DC Field | Value | Language |
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dc.contributor.author | Peixoto, Francisco | - |
dc.contributor.author | Vicente, Joaquim A. F. | - |
dc.contributor.author | Madeira, Vítor M. C. | - |
dc.date.accessioned | 2009-02-09T11:13:20Z | - |
dc.date.available | 2009-02-09T11:13:20Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Journal of Biochemical and Molecular Toxicology. 17:3 (2003) 185-192 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8147 | - |
dc.description.abstract | The herbicide dicamba (3,6-dichloro-2-methoxybenzoic acid) was evaluated for its effects on bioenergetic activities of potato tuber mitochondria to elucidate putative mechanisms of action and to compare its toxicity with 2-chlorobenzoic acid. Dicamba (4 mumol/mg mitochondrial protein) induces a limited stimulation of state 4 respiration of ca. 10%, and the above concentrations significantly inhibit respiration, whereas 2-chlorobenzoic acid maximally stimulates state 4 respiration (ca. 50%) at about 25 mumol/mg mitochondrial protein. As opposed to these limited effects on state 4 respiration, transmembrane electrical potential is strongly decreased by dicamba and 2-chlorobenzoic acid. Dicamba (25 mumol/mg mitochondrial protein) collapses, almost completely, Deltapsi; similar concentrations of 2-chlorobenzoic acid promote Deltapsi drops of about 50%. Proton permeabilization partially contributes to Deltapsi collapse since swelling in K-acetate medium is stimulated, with dicamba promoting a stronger stimulation. The Deltapsi decrease induced by dicamba is not exclusively the result of a stimulation on the proton leak through the mitochondrial inner membrane, since there was no correspondence between the Deltapsi decrease and the change on the O2 consumption on state 4 respiration; on the contrary, for concentrations above 8 mumol/mg mitochondrial protein a strong inhibition was observed. Both compounds inhibit the activity of respiratory complexes II and III but complex IV is not significantly affected. Complex I seems to be sensitive to these xenobiotics. In conclusion, dicamba is a stronger mitochondrial respiratory chain inhibitor and uncoupler as compared to 2-chlorobenzoic acid. Apparently, the differences in the lipophilicity are related to the different activities on mitochondrial bioenergetics. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:185-192, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10077 | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Comparative effects of herbicide dicamba and related compound on plant mitochondrial bioenergetics | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1002/jbt.10077 | en_US |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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