Title: Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
Authors: Ramos, F. 
Matos, A. 
Oliveira, A. 
Silveira, M. da 
Issue Date: 1999
Citation: Chromatographia. 50:1 (1999) 118-120
Abstract: Summary A method is described for the extraction of clebuterol from bovine retina samples by diphasic dialysis using gas chromatography—mass spectrometry (GC-MS) as the procedure of determination. Following chemical digestion of retinal tissue in alkaline medium at 80C, clenbuterol is extracted by diphasic dialysis using diethyl ether as solvent at 37C, for 4 h at 150 rpm. Diethyl ether is evaporated to dryness and clenbuterol determined by GC-MS after butylboronic acid (BBA) derivatization. A detection limit of 2.5 ng g-1, repeatability of 9.0% and reproducibility of 22.9%, with recoveries always >86% (n=5), are achieved as validation criteria.
URI: http://hdl.handle.net/10316/7939
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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