Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/7856
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dc.contributor.authorVieira, Margarida-
dc.contributor.authorPissarra, José-
dc.contributor.authorVeríssimo, Paula-
dc.contributor.authorCastanheira, Pedro-
dc.contributor.authorCosta, Yael-
dc.contributor.authorPires, Euclides-
dc.contributor.authorFaro, Carlos-
dc.date.accessioned2009-02-17T10:36:22Z-
dc.date.available2009-02-17T10:36:22Z-
dc.date.issued2001en_US
dc.identifier.citationPlant Molecular Biology. 45:5 (2001) 529-539en_US
dc.identifier.urihttp://hdl.handle.net/10316/7856-
dc.description.abstractCardosins A and B are related aspartic proteinases from the pistils of Cynara cardunculus L., whose milk-clotting activity has been exploited for the manufacture of cheese. Here we report the cloning of cardosin B cDNA and its organ, tissue and cytological localization. The cDNA-derived amino acid sequence has 73% similarity with that of cardosin A and displays several distinguishing features. Cardosin B mRNA was detected in young inflorescences but not in pistils of fully opened inflorescences, indicating that its expression is developmentally regulated. The proteinase, however, accumulates in the pistil until the later stages of floral development. Immunocytochemistry with a monospecific antibody localized cardosin B to the cell wall and extracellular matrix of the floral transmitting tissue. The location of cardosin B in the pistil is therefore clearly different from that of cardosin A, which was found at protein storage vacuoles of the stigmatic papillae and has been suggested to be involved in RGD-mediated proteolytic mechanisms. In view of these results the possible functions of cardosin B in the transmitting tissue are discussed.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleMolecular cloning and characterization of cDNA encoding cardosin B, an aspartic proteinase accumulating extracellularly in the transmitting tissue of Cynara cardunculus L.en_US
dc.typearticleen_US
dc.identifier.doi10.1023/A:1010675015318en_US
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-5853-0165-
crisitem.author.orcid0000-0002-0480-8379-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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