Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/7831
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dc.contributor.authorLuxo, Cristina-
dc.contributor.authorJurado, Amália-
dc.contributor.authorMadeira, Vítor-
dc.date.accessioned2009-02-17T10:36:41Z-
dc.date.available2009-02-17T10:36:41Z-
dc.date.issued2000en_US
dc.identifier.citationApplied Biochemistry and Biotechnology. 87:3 (2000) 219-232en_US
dc.identifier.urihttp://hdl.handle.net/10316/7831-
dc.description.abstractAbstract A strain of Bacillus stearothermophilus was used as a model to study physical perturbations induced in the membrane by the cytostatic tamoxifen (TAM). This study was carried out using two lines of criteria: (1) bacterial growth, and temperature growth range, with determination of growth parameters as a function of TAM concentration; and (2) biophysical studies by differential scanning calorimetry (DSC) and by means of two fluorescent probes to evaluate perturbations promoted by the drug on the structural order of bacterial lipid membranes. The inhibition of growth induced by TAM, the structural bilayer disordering, and the shift in the phase transition temperature to a lower range were also determined in the presence of Ca2+, i.e., a natural membrane stabilizer, to elucidate further perturbing effects of TAM on membranes with putative implications in cell toxicity. Growth inhibition promoted by TAM is potentiated by an increase in growth temperature above the optimal range, but attenuated or relieved by the addition of 2.5 mM Ca2+ to the culture medium. Consistently, fluorescence polarization and DSC studies showed that Ca2+ ions (2.5 mM) effectively compensated for the destabilizing effects promoted by TAM in bacterial lipid membranes.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleToxicity assessment of tamoxifen by means of a bacterial modelen_US
dc.typearticleen_US
dc.identifier.doi10.1385/ABAB:87:3:219en_US
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-7095-5337-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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