Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5856
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dc.contributor.authorAlves, Gilberto-
dc.contributor.authorFigueiredo, Isabel-
dc.contributor.authorCastel-Branco, Margarida-
dc.contributor.authorLoureiro, Ana-
dc.contributor.authorFalcão, Amílcar-
dc.contributor.authorCaramona, Margarida-
dc.date.accessioned2008-09-26T17:42:58Z-
dc.date.available2008-09-26T17:42:58Z-
dc.date.issued2007-
dc.identifier.citationAnalytica Chimica Acta. 596:1 (2007) 132-140-
dc.identifier.urihttp://hdl.handle.net/10316/5856-
dc.description.abstractHerein is reported, for the first time, a simple and reliable chiral reversed-phase liquid chromatographic method coupled to ultraviolet (UV) detection for simultaneous determination of eslicarbazepine acetate (ESL) and its metabolites, S-licarbazepine (S-LC), R-licarbazepine (R-LC) and oxcarbazepine (OXC), in mouse plasma and brain, liver and kidney tissue homogenates. All analytes and the internal standard were extracted from plasma and tissue homogenates by a solid-phase extraction procedure using Waters Oasis® hydrophilic-lipophilic balance cartridges. The chromatographic separation was performed by isocratic elution with water/methanol (88:12, v/v), pumped at a flow rate of 0.7 mL min-1, on a LichroCART 250-4 ChiraDex ([beta]-cyclodextrin, 5 [mu]m) column at 30 °C. The UV detector was set at 225 nm. Calibration curves were linear (r2 >= 0.996) in the ranges 0.4-8 [mu]g mL-1, 0.1-1.5 [mu]g mL-1 and 0.1-2 [mu]g mL-1 for ESL and OXC and in the ranges 0.4-80 [mu]g mL-1, 0.1-15 [mu]g mL-1 and 0.1-20 [mu]g mL-1 for R-LC and S-LC in plasma, brain and liver/kidney homogenates, respectively. The overall precision not exceeded 11.6% (%CV) and the accuracy ranged from -3.79 to 3.84% (%bias), considering all analytes in all matrices. Hence, this method will be a useful tool to characterize the pharmacokinetic disposition of ESL in mice.-
dc.description.urihttp://www.sciencedirect.com/science/article/B6TF4-4NWKCP8-6/1/b06d78493fb6a2c4fb6b5add5be30f0a-
dc.format.mimetypeaplication/PDF-
dc.language.isoeng-
dc.relationSFRH/12694/2003-
dc.rightsopenAccess-
dc.subjectEslicarbazepine acetate-
dc.subjectOxcarbazepine-
dc.subjectMouse tissue samples-
dc.subjectEnantioselective liquid chromatography-
dc.subjectBioanalytical method validation-
dc.titleSimultaneous and enantioselective liquid chromatographic determination of eslicarbazepine acetate, S-licarbazepine, R-licarbazepine and oxcarbazepine in mouse tissue samples using ultraviolet detection-
dc.typearticle-
degois.publication.firstPage132-
degois.publication.lastPage140-
degois.publication.issue1-
degois.publication.titleAnalytica Chimica Acta-
dc.date.updated2022-07-30T22:13:39Z-
dc.identifier.doi10.1016/j.aca.2007.05.056-
degois.publication.volume596-
dc.description.version3910-3178-31BA | MARIA MARGARIDA COUTINHO DE SEABRA CASTEL-BRANCO CAETANO-
dc.description.versioninfo:eu-repo/semantics/publishedVersion-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculty of Pharmacy-
crisitem.author.deptFaculty of Pharmacy-
crisitem.author.deptFaculty of Pharmacy-
crisitem.author.deptFaculty of Pharmacy-
crisitem.author.researchunitICBR Coimbra Institute for Clinical and Biomedical Research-
crisitem.author.parentresearchunitFaculty of Medicine-
crisitem.author.orcid0000-0002-6533-9932-
crisitem.author.orcid0000-0002-3854-6549-
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