Title: Lamotrigine analysis in blood and brain by high-performance liquid chromatography
Authors: Castel-Branco, M. M. 
Almeida, A. M. 
Falcão, Amílcar 
Macedo, T. A. 
Caramona, M. M. 
Lopez, F. G. 
Keywords: Lamotrigine
Issue Date: 2001
Citation: Journal of Chromatography B: Biomedical Sciences and Applications. 755:1-2 (2001) 119-127
Abstract: A reversed-phase high-performance liquid chromatography assay was developed and validated to determine plasma and brain lamotrigine concentrations allowing pharmacokinetic-pharmacodynamic studies of this new antiepileptic drug in patients and laboratory animals. Lamotrigine and its internal standard were extracted, under alkaline conditions, from plasma and brain homogenate, into ethyl acetate; brain proteins were previously precipitated with trichloroacetic acid. The method was linear between 0.1 and 15.0 mg/l for plasma, with a detection limit of 0.008 mg/l, and between 0.1 and 5.0 mg/l for brain homogenate, with a detection limit of 0.023 mg/l. The method proved to be simple, useful and appropriate, not only for clinical and experimental research, but also for routine monitoring of lamotrigine concentrations in patients.
URI: http://hdl.handle.net/10316/5803
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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