Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5744
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dc.contributor.authorBorges, Olga-
dc.contributor.authorCordeiro-da-Silva, Anabela-
dc.contributor.authorRomeijn, Stefan G.-
dc.contributor.authorAmidi, Maryam-
dc.contributor.authorSousa, Adriano de-
dc.contributor.authorBorchard, Gerrit-
dc.contributor.authorJunginger, Hans E.-
dc.date.accessioned2008-09-26T17:40:58Z-
dc.date.available2008-09-26T17:40:58Z-
dc.date.issued2006en_US
dc.identifier.citationJournal of Controlled Release. 114:3 (2006) 348-358en_US
dc.identifier.urihttp://hdl.handle.net/10316/5744-
dc.description.abstractThe design of particulate vaccine delivery systems, particularly for mucosal surfaces, has been a focus of interest in recent years. In this context, we have previously described the development and the characterization of a new nanosized delivery system, consisting of a model antigen adsorbed to chitosan particles and coated with sodium alginate. In the present work the ovalbumin release profiles from these coated nanoparticles in different pH buffers were investigated and compared to those of the uncoated particles. Cytotoxicity of the polymers and nanoparticles was assessed using the MTT assay. Finally, particle uptake studies in rat Peyer's patches were performed. It was demonstrated that the coating of the nanoparticles with sodium alginate not only avoided a burst release observed with uncoated particles but also increased the stability of the particles at pH 6.8 and 7.4 at 37 °C. At neutral pH, the release was lower than 5% after 3.5 h incubation in a low ionic strength buffer. For both, chitosan and alginate polymers, and for the nanoparticles, comparable cell viability data close to 100%, were obtained. Additionally, based on confocal laser scanning microscopy observations, it was shown that alginate coated nanoparticles were able to be taken up by rat Peyer's patches, rendering them suitable carriers for intestinal mucosal vaccination.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T3D-4K6CHRP-1/1/a257a285900ecde6d4747e1df67e1a82en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCoated nanoparticlesen_US
dc.subjectChitosanen_US
dc.subjectSodium alginateen_US
dc.subjectPeyer's patchesen_US
dc.subjectCytotoxicityen_US
dc.titleUptake studies in rat Peyer's patches, cytotoxicity and release studies of alginate coated chitosan nanoparticles for mucosal vaccinationen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.jconrel.2006.06.011-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
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