Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5468
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gonçalves, P. P. | - |
dc.contributor.author | Meireles, S. M. | - |
dc.contributor.author | Gravato, C. | - |
dc.contributor.author | Vale, M. G. P. | - |
dc.date.accessioned | 2008-09-01T15:42:48Z | - |
dc.date.available | 2008-09-01T15:42:48Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Neuroscience Letters. 247:2-3 (1998) 87-90 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/5468 | - |
dc.description.abstract | Synaptic vesicles isolated from sheep brain cortex exhibit an ATP-dependent Ca2+ accumulation that is inhibited by the protonophore uncoupler carbonyl cyanide m-chorophenylhydrazone (CCCP) and completely released by the Ca2+ ionophore ionomycin. This transport activity was sensitive to the V-type ATPase inhibitor, bafilomycin, but not to the P-type ATPase inhibitor, vanadate. We also observed that the proton gradient, established across the synaptic vesicle membranes in the presence of ATP, is partially dissipated by the addition of Ca2+ (100-860 [mu]M) in correlation to an increase of ATP hydrolysis by the H+-pumping ATPase. In contrast, the activity of the H+-ATPase, measured under uncoupling conditions (presence of CCCP), appears to be unaltered by the calcium ion. The Ca2+-induced H+ release visualized by fluorescence quenching of acridine orange correlates well with the Ca2+ uptake determined isotopically. These results indicate that synaptic vesicles accumulate Ca2+, via a low affinity Ca2+-H+ antiport system energized by the protonmotive force originated from the H+-pumping ATPase activity. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T0G-3STG33F-M/1/23212cde5694bae6fc9101190baa18ed | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Synaptic vesicles | en_US |
dc.subject | Ca2+ transport | en_US |
dc.subject | Ca2+-H+ antiport | en_US |
dc.title | Ca2+-H+ antiport activity in synaptic vesicles isolated from sheep brain cortex | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S0304-3940(98)00256-0 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
fileab9d52755db44890ae2cd5359c79fb50.pdf | 149.55 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
28
checked on Apr 22, 2024
WEB OF SCIENCETM
Citations
26
checked on Apr 2, 2024
Page view(s)
298
checked on Apr 16, 2024
Download(s) 50
581
checked on Apr 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.