Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/5468
Título: | Ca2+-H+ antiport activity in synaptic vesicles isolated from sheep brain cortex | Autor: | Gonçalves, P. P. Meireles, S. M. Gravato, C. Vale, M. G. P. |
Palavras-chave: | Synaptic vesicles; Ca2+ transport; Ca2+-H+ antiport | Data: | 1998 | Citação: | Neuroscience Letters. 247:2-3 (1998) 87-90 | Resumo: | Synaptic vesicles isolated from sheep brain cortex exhibit an ATP-dependent Ca2+ accumulation that is inhibited by the protonophore uncoupler carbonyl cyanide m-chorophenylhydrazone (CCCP) and completely released by the Ca2+ ionophore ionomycin. This transport activity was sensitive to the V-type ATPase inhibitor, bafilomycin, but not to the P-type ATPase inhibitor, vanadate. We also observed that the proton gradient, established across the synaptic vesicle membranes in the presence of ATP, is partially dissipated by the addition of Ca2+ (100-860 [mu]M) in correlation to an increase of ATP hydrolysis by the H+-pumping ATPase. In contrast, the activity of the H+-ATPase, measured under uncoupling conditions (presence of CCCP), appears to be unaltered by the calcium ion. The Ca2+-induced H+ release visualized by fluorescence quenching of acridine orange correlates well with the Ca2+ uptake determined isotopically. These results indicate that synaptic vesicles accumulate Ca2+, via a low affinity Ca2+-H+ antiport system energized by the protonmotive force originated from the H+-pumping ATPase activity. | URI: | https://hdl.handle.net/10316/5468 | DOI: | 10.1016/S0304-3940(98)00256-0 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
fileab9d52755db44890ae2cd5359c79fb50.pdf | 149.55 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
28
Visto em 22/abr/2024
Citações WEB OF SCIENCETM
26
Visto em 2/abr/2024
Visualizações de página
298
Visto em 16/abr/2024
Downloads 50
581
Visto em 16/abr/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.