Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5468
Title: Ca2+-H+ antiport activity in synaptic vesicles isolated from sheep brain cortex
Authors: Gonçalves, P. P. 
Meireles, S. M. 
Gravato, C. 
Vale, M. G. P. 
Keywords: Synaptic vesicles; Ca2+ transport; Ca2+-H+ antiport
Issue Date: 1998
Citation: Neuroscience Letters. 247:2-3 (1998) 87-90
Abstract: Synaptic vesicles isolated from sheep brain cortex exhibit an ATP-dependent Ca2+ accumulation that is inhibited by the protonophore uncoupler carbonyl cyanide m-chorophenylhydrazone (CCCP) and completely released by the Ca2+ ionophore ionomycin. This transport activity was sensitive to the V-type ATPase inhibitor, bafilomycin, but not to the P-type ATPase inhibitor, vanadate. We also observed that the proton gradient, established across the synaptic vesicle membranes in the presence of ATP, is partially dissipated by the addition of Ca2+ (100-860 [mu]M) in correlation to an increase of ATP hydrolysis by the H+-pumping ATPase. In contrast, the activity of the H+-ATPase, measured under uncoupling conditions (presence of CCCP), appears to be unaltered by the calcium ion. The Ca2+-induced H+ release visualized by fluorescence quenching of acridine orange correlates well with the Ca2+ uptake determined isotopically. These results indicate that synaptic vesicles accumulate Ca2+, via a low affinity Ca2+-H+ antiport system energized by the protonmotive force originated from the H+-pumping ATPase activity.
URI: http://hdl.handle.net/10316/5468
DOI: 10.1016/S0304-3940(98)00256-0
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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