Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/5465
DC FieldValueLanguage
dc.contributor.authorGonçalves, Paula P.-
dc.contributor.authorMeireles, Sandra M.-
dc.contributor.authorNeves, Paulo-
dc.contributor.authorVale, M. Graça P.-
dc.date.accessioned2008-09-01T15:42:45Z-
dc.date.available2008-09-01T15:42:45Z-
dc.date.issued1999en_US
dc.identifier.citationMolecular Brain Research. 67:2 (1999) 283-291en_US
dc.identifier.urihttps://hdl.handle.net/10316/5465-
dc.description.abstractAs we previously reported, synaptic vesicles isolated from sheep brain cortex contain a Ca2+/H+ antiport that permits Ca2+ accumulation inside the vesicles (~5 nmol/mg protein) at expenses of the pH gradient generated by the H+-pumping ATPase. We observed that the system associates Ca2+ influx to H+ release and operates with low affinity for Ca2+. In the present work, we found that Ca2+/H+ antiport mediates exchange of protons with other cations such as Zn2+ and Cd2+, suggesting that these cations and Ca2+ share the same transporter molecules to enter the intravesicular space. Zn2+ and Cd2+ induce H+ release in a concentration-dependent manner (fluorimetrically evaluated) and they inhibit the antiport-mediated Ca2+ uptake by the vesicles (isotopically measured). In contrast, large cations such as Ba2+ and Cs+ do not alter Ca2+ influx and they are unable to induce proton release from the vesicles. With respect to Sr2+, which has an intermediary size relatively to the other groups of cations, we found that it does not induce H+ liberation from the vesicles, but it has a concentration-dependent inhibitory effect on the Ca2+-induced H+ release and Ca2+ uptake by the vesicles. These results indicate that the cation selectivity of the synaptic vesicles Ca2+/H+ antiport is essentially determined by the size of the cation transported into the vesicles.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T07-3W9CWTS-9/1/5dba905676aadcbf1c390548cc657219en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCations transporten_US
dc.subjectCa2+/H+ antiporten_US
dc.subjectSynaptic vesicleen_US
dc.subjectBrain cortexen_US
dc.titleIonic selectivity of the Ca2+/H+ antiport in synaptic vesicles of sheep brain cortexen_US
dc.typearticleen_US
dc.identifier.doi10.1016/S0169-328X(99)00081-9-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
file27376450abe84262aaef2a5ab0a7a665.pdf392.68 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

25
checked on Apr 1, 2024

WEB OF SCIENCETM
Citations

21
checked on Apr 2, 2024

Page view(s)

206
checked on Apr 16, 2024

Download(s)

204
checked on Apr 16, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.