Effects of NADH and H2O2 on Chromate-Induced Human Erythrocytes Hemoglobin Oxidation and Peroxidation

Abstract

The effects of NADH and H2O2 on chromate-induced human erythrocyte hemoglobin oxidation and peroxidation were studied. It was observed that NADH decreases the levels of chromate-induced human erythrocyte hemoglobin oxidation and peroxidation. H2O2 decreases the levels of chromate-induced hemoglobin oxidation, but increases the levels of chromate-induced peroxidation. The ability of H2O2 to decrease the levels of chromate-induced hemoglobin oxidation is higher than that observed for NADH. Furthermore, H2O2 increases the inhibitory effect of NADH on chromate-induced hemoglobin oxidation, but decreases the NADH effect on chromate-induced peroxidation. The meaning of these results is discussed in terms of involvement of reactive chromium(V) species and reactive oxygen species in the mechanism by which chromate induces its effects in human erythrocytes.