Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5400
DC FieldValueLanguage
dc.contributor.authorDuarte, Ana Isabel-
dc.contributor.authorSantos, Maria Sancha-
dc.contributor.authorSeiça, Raquel-
dc.contributor.authorOliveira, Catarina Resende de-
dc.date.accessioned2008-09-01T15:41:38Z-
dc.date.available2008-09-01T15:41:38Z-
dc.date.issued2003en_US
dc.identifier.citationBrain Research. 977:1 (2003) 23-30en_US
dc.identifier.urihttp://hdl.handle.net/10316/5400-
dc.description.abstractIn this study, we investigated the in vitro effect of exogenously administered insulin on the susceptibility to oxidative stress and on the accumulation of the amino acid neurotransmitters [gamma]-aminobutyric acid (GABA) and glutamate in a synaptosomal fraction isolated from male Wistar rat brain cortex. Insulin (1 [mu]M) did not affect synaptosomal lipid peroxidation induced by the oxidant pair ascorbate/Fe2+, although under these conditions an increase in thiobarbituric acid reactive substances (TBARS) levels was observed. Under control conditions, the presence of insulin did not change the uptake of [3H]GABA or [3H]glutamate. In contrast, under oxidizing conditions, we observed a 1.8- and a 2.2-fold decrease in [3H]GABA and [3H]glutamate accumulation, respectively, and insulin reverted the lower levels of both [3H]GABA and [3H]glutamate accumulation (to 86.74±6.26 and 67.01±6.65% of control, respectively). Insulin also increased the extrasynaptosomal levels of GABA and glutamate, determined both in control and oxidizing conditions. From this study, we can conclude that insulin is a modulator of amino acid neurotransmitter transport, either directly, as seems to occur under normal conditions, or via the decrease in ATP levels and the subsequent reversion of the amino acid transporters, as seems to occur under oxidative stress conditions. The modulation of both GABA and glutamate transport might be implicated in the neuroprotective role of insulin.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6SYR-48GPC08-1/1/05072ca679697e5fe295ce2150d051caen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectInsulinen_US
dc.subjectGlutamateen_US
dc.subjectOxidative stressen_US
dc.subjectRat brain synaptosomeen_US
dc.titleInsulin affects synaptosomal GABA and glutamate transport under oxidative stress conditionsen_US
dc.typearticleen_US
dc.identifier.doi10.1016/S0006-8993(03)02679-9-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-6881-9392-
crisitem.author.orcid0000-0001-6942-4328-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
file5750f85cf57447c0aa4d219727e1bbf5.pdf202.33 kBAdobe PDFView/Open
Show simple item record

WEB OF SCIENCETM
Citations

25
checked on Oct 2, 2021

Page view(s) 20

655
checked on Oct 12, 2021

Download(s)

180
checked on Oct 12, 2021

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.