Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5333
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dc.contributor.authorSantiago, A. R.-
dc.contributor.authorCristóvão, A. J.-
dc.contributor.authorSantos, P. F.-
dc.contributor.authorCarvalho, C. M.-
dc.contributor.authorAmbrósio, A. F.-
dc.date.accessioned2008-09-01T15:40:27Z-
dc.date.available2008-09-01T15:40:27Z-
dc.date.issued2007en_US
dc.identifier.citationNeurobiology of Disease. 25:3 (2007) 464-472en_US
dc.identifier.urihttp://hdl.handle.net/10316/5333-
dc.description.abstractDiabetic retinopathy is a leading cause of blindness among adults in the western countries. It has been reported that neurodegeneration may occur in diabetic retinas, but the mechanisms underlying retinal cell death are poorly understood. We found that high glucose increased the number of cells with condensed nuclei and the number of TUNEL-positive cells, and caused an increase in the translocation of phosphatidylserine to the outer leaflet of the plasma membrane, indicating that high glucose induces apoptosis in cultured retinal neural cells. The activity of caspases did not increase in high glucose-treated cells, but apoptosis-inducing factor (AIF) levels decreased in the mitochondria and increased in the nucleus, indicating a translocation to the nucleus where it may cause DNA fragmentation. These results demonstrate that elevated glucose induces apoptosis in cultured retinal neural cells. The increase in apoptosis is not dependent on caspase activation, but is mediated through AIF release from the mitochondria.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6WNK-4MKCGWG-1/1/0b1383d4de478900bac3fea0f32aa802en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectApoptosisen_US
dc.subjectMitochondriaen_US
dc.subjectRetinopathyen_US
dc.subjectApoptosis-inducing factor (AIF)en_US
dc.subjectDiabetesen_US
dc.titleHigh glucose induces caspase-independent cell death in retinal neural cellsen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.nbd.2006.10.023-
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-7541-7041-
crisitem.author.orcid0000-0002-4090-3269-
crisitem.author.orcid0000-0002-0477-1641-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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