Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5143
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dc.contributor.authorPiedade, J. A. P.-
dc.contributor.authorMano, M.-
dc.contributor.authorLima, M. C. Pedroso de-
dc.contributor.authorOretskaya, T. S.-
dc.contributor.authorOliveira-Brett, A. M.-
dc.date.accessioned2008-09-01T15:04:41Z-
dc.date.available2008-09-01T15:04:41Z-
dc.date.issued2004-
dc.identifier.citationJournal of Electroanalytical Chemistry. 564:(2004) 25-34en_US
dc.identifier.urihttp://hdl.handle.net/10316/5143-
dc.description.abstractThe voltammetric behaviour of oligonucleotide lipoplexes (ODN-lipoplexes) prepared from short oligodeoxynucleotides (ODN), with different base compositions, and liposomes of the cationic lipid DOTAP, was studied by differential pulse voltammetry with a glassy carbon mini-electrode. It was found that the ODN base composition influences the ODN-lipoplex voltammetric response. Differential pulse voltammograms for ODN-lipoplexes of the ODN adenosine nucleotides present two different features when compared with the differential pulse voltammograms obtained for free ODN: a new peak appeared and the peak attributed to oxidation of adenosine diminished or was absent, depending on whether the ODN sequence had guanosine nucleotides or not. The presence of guanosine nucleotides in the ODN-lipoplex led to a peak due to guanosine oxidation with similar potential and current to the peak obtained for guanosine oxidation in free ODN. No detectable peaks were recorded in the voltammograms obtained with lipoplexes composed of ODN containing only pyrimidine bases. It was possible to show by voltammetry the occurrence of partial denaturation of short double helices of ODN when mixed with DOTAP liposomes to generate lipoplexes. The extent of denaturation was observed to increase with lipoplex (+/-) charge ratio as shown by the increase in the differential pulse voltammetry peak currents. The electrochemical characterisation of lipoplex properties at a charged interface can be important for understanding and development of these gene therapy vectors.pt
dc.description.urihttp://www.sciencedirect.com/science/article/B6TGB-4B4RVT3-2/1/80478920c615683b2439ef8a29808980en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengpt
dc.rightsopenAccesspt
dc.subjectVoltammetrypt
dc.subjectElectrified interfacept
dc.subjectLipoplexpt
dc.subjectCationic liposomept
dc.subjectOligonucleotidept
dc.titleVoltammetric behaviour of oligonucleotide lipoplexes adsorbed onto glassy carbon electrodespt
dc.typearticleen_US
dc.peerreviewedyespt
dc.identifier.doi10.1016/j.jelechem.2003.10.008pt
dc.date.embargo2004-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.orcid0000-0003-1922-4824-
crisitem.author.orcid0000-0003-1844-5027-
crisitem.author.orcid0000-0002-6244-0891-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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