Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5084
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dc.contributor.authorPaquim, Ana Maria Chiorcea-
dc.contributor.authorOretskaya, Tatiana S.-
dc.contributor.authorBrett, Ana Maria Oliveira-
dc.date.accessioned2008-09-01T15:03:40Z-
dc.date.available2008-09-01T15:03:40Z-
dc.date.issued2006en_US
dc.identifier.citationBiophysical Chemistry. 121:2 (2006) 131-141en_US
dc.identifier.urihttp://hdl.handle.net/10316/5084-
dc.description.abstractDNA adsorption on electrode surfaces is of fundamental interest for the development of DNA-based biosensors. The free adsorption of 10-mer synthetic oligodeoxynucleotides (ODNs) onto highly oriented pyrolytic graphite (HOPG) surfaces was studied using Magnetic AC mode atomic force microscopy (MAC Mode AFM). The mechanism of interaction of nucleic acids with carbon electrode surfaces was elucidated, using 10-mer synthetic homo- and hetero-ODNs sequences of known base sequences, because they allow clear interpretation of the experimental data. AFM images in air revealed different adsorption patterns and degree of HOPG surface coverage for the ODNs, and correlation with the individual structure and base sequence of each ODN molecule will be presented. The results demonstrated that the hydrophobic interactions with the HOPG hydrophobic surface explain the main adsorption mechanism, although other effects such as electrostatic and Van der Waals interactions may contribute to the free adsorption process. The ODNs interacted differently with the HOPG surface, according to the ODN sequence hydrophobic characteristics, being directly depending on the molecular mass, the hydrophobic character of the individual bases and on the secondary structure of the molecule. The importance of the type of base existent at the ODN chain extremities on the adsorption process was investigated and different adsorption patterns were obtained with ODN sequences composed by the same group of bases aligned in a different order.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6TFB-4J6WR05-2/1/64ef57ec21856f05e10206d1d7b10334en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectOligodeoxynucleotidesen_US
dc.subjectAdsorptionen_US
dc.subjectDNA-biosensoren_US
dc.subjectHydrophobic interactionsen_US
dc.titleAdsorption of synthetic homo- and hetero-oligodeoxynucleotides onto highly oriented pyrolytic graphite: Atomic force microscopy characterizationen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.bpc.2005.12.017-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.orcid0000-0002-0605-5147-
crisitem.author.orcid0000-0002-6244-0891-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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