Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/5080
DC FieldValueLanguage
dc.contributor.authorKramer-Marek, Gabriela-
dc.contributor.authorSerpa, Carlos-
dc.contributor.authorSzurko, Agnieszka-
dc.contributor.authorWidel, Maria-
dc.contributor.authorSochanik, Aleksander-
dc.contributor.authorSnietura, Miroslaw-
dc.contributor.authorKus, Piotr-
dc.contributor.authorNunes, Rui M. D.-
dc.contributor.authorArnaut, Luís G.-
dc.contributor.authorRatuszna, Alicja-
dc.date.accessioned2008-09-01T15:03:36Z-
dc.date.available2008-09-01T15:03:36Z-
dc.date.issued2006en_US
dc.identifier.citationJournal of Photochemistry and Photobiology B: Biology. 84:1 (2006) 1-14en_US
dc.identifier.urihttps://hdl.handle.net/10316/5080-
dc.description.abstractPhotodynamic therapy (PDT) and photodynamic diagnostics (PDD) of cancer are based on the use of non-toxic dyes (photosensitisers) in combination with harmless visible light. This paper reports physicochemical properties, cell uptake, localisation as well as photodynamic efficiency of two novel lipophilic porphyrin derivatives, suitable for use as PDT sensitisers. Both compounds are characterised by high quantum yield of singlet oxygen generation which was measured by time-resolved phosphorescence. Photodynamic in vitro studies were conducted on three cancer cell lines. Results of cell survival tests showed negligible dark cytotoxicity but high phototoxicity. The results also indicate that cell death is dependent on energy dose and time following light exposure. Using confocal laser scanning microscopy both compounds were found to localise in the cytoplasm around the nucleus of the tumour cells. The mode of cell death was evaluated based on the morphological changes after differential staining.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6TH0-4J9X306-1/1/a3dd4e8191b4f273644ef70f2a3cdbccen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPhotodynamic therapyen_US
dc.subjectSinglet oxygenen_US
dc.subjectPhotosensitisationen_US
dc.subjectApoptosisen_US
dc.subjectNecrosisen_US
dc.titleSpectroscopic properties and photodynamic effects of new lipophilic porphyrin derivatives: Efficacy, localisation and cell death pathwaysen_US
dc.typearticleen_US
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0001-7004-0110-
crisitem.author.orcid0000-0002-3223-4819-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
file3fb912e70e6e4651837df17dd70f1736.pdf1.47 MBAdobe PDFView/Open
Show simple item record

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.