Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/48397
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ventura, Célia | - |
dc.contributor.author | Lourenço, Ana Filipa | - |
dc.contributor.author | Sousa-Uva, António | - |
dc.contributor.author | Ferreira, Paulo J.T. | - |
dc.contributor.author | Silva, Maria João | - |
dc.date.accessioned | 2018-04-19T16:43:20Z | - |
dc.date.issued | 2018-04 | - |
dc.identifier.citation | Ventura, C., Lourenço, A. F., Sousa-Uva, A. Ferreira, P. J., Silva, M.J. (2018). Evaluating the Genotoxicity of Cellulose Nanofibrils in a Co-Culture of Human Lung Epithelial Cells and Monocyte-derived Macrophages. Toxicology Letters. 10.1016/j.toxlet.2018.04.013 | en |
dc.identifier.issn | 0378-4274 | pt |
dc.identifier.issn | ESSN: 1879-3169 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/48397 | - |
dc.description.abstract | Cellulose nanofibrils (CNF) are manufactured nanofibres that hold impressive expectations in forest, food, pharmaceutical, and biomedical industries. CNF production and applications are leading to an increased human exposure and thereby it is of utmost importance to assess its safety to health. In this study, we screened the cytotoxic, immunotoxic and genotoxic effects of a CNF produced by TEMPO-mediated oxidation of an industrial bleached Eucalyptus globulus kraft pulp on a co-culture of lung epithelial alveolar (A549) cells and monocyte-derived macrophages (THP-1 cells). The results indicated that low CNF concentrations can stimulate A549 cells proliferation, whereas higher concentrations are moderately toxic. Moreover, no proinflammatory cytokine IL- was detected in the co-culture medium suggesting no immunotoxicity. Although CNF treatment did not induce sizable levels of DNA damage in A549 cells, it leaded to micronuclei formation at 1.5 and 3 μg/cm2. These findings suggest that this type of CNF is genotoxic through aneugenic or clastogenic mechanisms. Noteworthy, cell overgrowth and genotoxicity, which are events relevant for cell malignant transformation, were observed at low CNF concentration levels, which are more realistic and relevant for human exposure, e.g., in occupational settings. | pt |
dc.language.iso | eng | pt |
dc.publisher | Elsevier | pt |
dc.rights | embargoedAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt |
dc.subject | Cellulose nanofibrils | pt |
dc.subject | Safety assessment | pt |
dc.subject | Immunotoxicity | pt |
dc.subject | Comet assay | pt |
dc.subject | Micronucleus assay | pt |
dc.title | Evaluating the Genotoxicity of Cellulose Nanofibrils in a Co-Culture of Human Lung Epithelial Cells and Monocyte-derived Macrophages | pt |
dc.type | article | - |
dc.relation.publisherversion | 10.1016/j.toxlet.2018.04.013 | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1016/j.toxlet.2018.04.013 | pt |
dc.date.embargo | 2018-04-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
crisitem.author.researchunit | CIEPQPF – Chemical Process Engineering and Forest Products Research Centre | - |
crisitem.author.researchunit | CIEPQPF – Chemical Process Engineering and Forest Products Research Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0002-2860-9833 | - |
crisitem.author.orcid | 0000-0002-4503-6811 | - |
crisitem.author.orcid | 0000-0002-6060-0716 | - |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Evaluating the genotoxicity of cellulose nanofibrils in a co-culture of human lung_Accepted_Manuscript.pdf | 3.57 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
36
checked on Apr 15, 2024
WEB OF SCIENCETM
Citations
20
30
checked on Apr 2, 2024
Page view(s)
318
checked on Apr 16, 2024
Download(s)
600
checked on Apr 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License