Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/4798
DC FieldValueLanguage
dc.contributor.authorAlmeida, T.-
dc.contributor.authorRodrigues, R. J.-
dc.contributor.authorMendonça, A. de-
dc.contributor.authorRibeiro, J. A.-
dc.contributor.authorCunha, R. A.-
dc.date.accessioned2008-09-01T14:14:39Z-
dc.date.available2008-09-01T14:14:39Z-
dc.date.issued2003en_US
dc.identifier.citationNeuroscience. 122:1 (2003) 111-121en_US
dc.identifier.urihttps://hdl.handle.net/10316/4798-
dc.description.abstractElectrophysiological recordings were used to investigate the effects of ATP analogues on [theta]-burst-induced long-term potentiation (LTP) in rat hippocampal slices. [alpha],[beta]-Methylene ATP ([alpha],[beta]-MeATP; 20 [mu]M) decreased LTP from 36±9% to 17±5%, an effect prevented by adenosine A1 receptor blockade in accordance with the localised catabolism of ATP analogues into adenosine, leading to adenosine A1 receptor activation. Thus, to probe the role of extracellular ATP, all experiments were performed with the A1 receptor selective antagonist, 1,3-dipropyl-8-cyclopentylxanthine (50 nM). In these conditions, [alpha],[beta]-MeATP or 5'-adenylylimido-diphosphate ([beta],[gamma]-ImATP; 20 [mu]M) facilitated LTP by 120%, an effect prevented by the P2 receptor antagonists, pyridoxalphosphate-6-azophenyl-2'-4'-disulphonic acid (PPADS; 20 [mu]M) or suramin (75 [mu]M), as well as by the P2X1/3-selective antagonist 8-(benzamido)naphthalene-1,3,5-trisulfonate (10 [mu]M). The facilitations of LTP by either [alpha],[beta]-MeATP or [beta],[gamma]-ImATP (20 [mu]M) were also prevented by both 4-(2-[7-amino-2-(2-furyl(1,2,4)-triazolo(2,3a)-(1,3,5)triazin-5-yl-amino]ethyl)phenol (50 nM) or 7-2(-phenylethyl)-5-amino-2-(2-furyl)-pyrazolo-[4,3-e]-1,2,4-triazolo[1,5-c] pyrimidine (50 nM), antagonists of facilitatory adenosine A2A receptors, were occluded by the A2A receptor agonist, CGS 21680 (10 nM) and were prevented by the protein kinase C inhibitor, chelerythrine (6 [mu]M) and unaffected by the protein kinase A inhibitor, H89 (1 [mu]M). Furthermore, [beta],[gamma]-ImATP (20 [mu]M) enhanced [3H]adenosine outflow from rat hippocampal slices by nearly 150%, an effect prevented by PPADS (20 [mu]M) or suramin (75 [mu]M). The adenosine transport inhibitors, nitrobenzylthioinosine (5 [mu]M) and dipyridamole (10 [mu]M) also prevented [beta],[gamma]-ImATP (20 [mu]M)-induced [3H]adenosine outflow and facilitation of LTP. These results suggest that ATP analogues facilitate LTP through P2 receptor activation that mainly triggers adenosine release leading to the activation of adenosine A2A receptors.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T0F-49SFDNY-1/1/bd15c287ddd50f5af9c1be35dcb92275en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectneuromodulationen_US
dc.subjecthippocampusen_US
dc.titlePurinergic P2 receptors trigger adenosine release leading to adenosine A2A receptor activation and facilitation of long-term potentiation in rat hippocampal slicesen_US
dc.typearticleen_US
dc.identifier.doi10.1016/S0306-4522(03)00523-2-
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0003-2550-6422-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
file1c4b85578e1642bc96be9156ae780053.pdf366.02 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

52
checked on Mar 25, 2024

WEB OF SCIENCETM
Citations

47
checked on Mar 2, 2024

Page view(s)

369
checked on Mar 26, 2024

Download(s)

331
checked on Mar 26, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.