Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/4720
Título: | Evaluation of the antitumoral effect mediated by IL-12 and HSV-tk genes when delivered by a novel lipid-based system | Autor: | Faneca, H. Cabrita, A. S. Simões, S. Lima, M. C. Pedroso de |
Palavras-chave: | Cancer gene therapy; "Suicide" gene therapy; Immunotherapy; Gene delivery; Cationic liposomes; Transfection | Data: | 2007 | Citação: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1768:5 (2007) 1093-1102 | Resumo: | In the present work, we used a novel albumin-associated lipoplex formulation, containing the cationic lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC) and cholesterol (Chol), to evaluate the antitumoral efficacy of two gene therapy strategies: immuno-gene therapy, mediated by IL-12 gene expression, and "suicide" gene therapy, mediated by HSV-tk gene expression followed by ganciclovir (GCV) treatment. Our data show that, in an animal model bearing a subcutaneous TSA (mouse mammary adenocarcinoma) tumor, intratumoral administration of the albumin-associated complexes containing the plasmid encoding IL-12 results in a strong antitumoral effect, as demonstrated by the smaller tumor size, the higher T-lymphocyte tumor infiltration and the more extensive tumor necrotic and hemorrhagic areas, as compared to that observed in animals treated with control complexes. On the other hand, the application of the "suicide" gene therapy strategy results in a significant antitumoral activity, which is similar to that achieved with the immuno-gene therapy strategy, although involving different antineoplastic mechanisms. For the tested model, albumin-associated complexes were shown to efficiently mediate intratumoral delivery of therapeutic genes, thus leading to a significant antitumoral effect. This finding is particularly relevant since TSA tumors are characterized for being poorly immunogenic, aggressive and exhibiting high proliferation capacity. | URI: | https://hdl.handle.net/10316/4720 | DOI: | 10.1016/j.bbamem.2006.12.017 | Direitos: | openAccess |
Aparece nas coleções: | FMUC Medicina - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
file4e13d68bfdb645a3a6a16feab7dcf0eb.pdf | 942.52 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
27
Visto em 15/abr/2024
Citações WEB OF SCIENCETM
22
Visto em 2/abr/2024
Visualizações de página 50
576
Visto em 16/abr/2024
Downloads
284
Visto em 16/abr/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.