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dc.contributor.authorSilva, Carla G.en_US
dc.contributor.authorPorciúncula, Lisiane O.en_US
dc.contributor.authorCanas, Paula M.en_US
dc.contributor.authorOliveira, Catarina R.en_US
dc.contributor.authorCunha, Rodrigo A.en_US
dc.date.accessioned2008-09-01T14:13:12Z-
dc.date.available2008-09-01T14:13:12Z-
dc.date.issued2007en_US
dc.identifier.citationNeurobiology of Disease. 27:2 (2007) 182-189en_US
dc.identifier.urihttp://hdl.handle.net/10316/4713-
dc.description.abstractSince adenosine A2A receptor (A2ARs) blockade protects against noxious brain insults involving apoptosis, we directly tested if A2AR blockade prevents apoptosis induced by staurosporine (STS). Exposure of rat hippocampal neurons to STS (30 nM, 24 h) decreased neuronal viability while increasing the number apoptotic-like neurons and de-localizing mitochondria and cytochrome c immunoreactivities. This was prevented by the selective A2AR antagonists, SCH58261 and ZM241385 (50 nM). Shorter incubation periods (6 h) with STS caused no neuronal loss but decreased synaptophysin and MAP-2 immunoreactivities, which was prevented by SCH58261. Furthermore, STS (100 nM) decreased MTT reduction and increased caspase-3 activity in rat hippocampal nerve terminals, which was prevented by SCH58261. These results show that A2AR blockade inhibits STS-induced apoptotic-like neuronal cell death. This begins with an apoptotic-like synaptotoxicity, which later evolved into an overt neurotoxicity, and A2ARs effectively control this initial synaptotoxicity, in agreement with their predominant synaptic localization in the hippocampus.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6WNK-4NTRT29-1/1/e8601014d0729171e70d9df284a40442en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAdenosineen_US
dc.subjectA2A receptoren_US
dc.subjectStaurosporineen_US
dc.subjectApoptosisen_US
dc.subjectNeuroprotectionen_US
dc.subjectMitochondriaen_US
dc.subjectHippocampusen_US
dc.subjectCultured neuronsen_US
dc.subjectSynaptosomesen_US
dc.subjectCaspase-3en_US
dc.titleBlockade of adenosine A2A receptors prevents staurosporine-induced apoptosis of rat hippocampal neuronsen_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.grantfulltextopen-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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