Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/4705
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dc.contributor.authorReis, F.-
dc.contributor.authorRocha-Pereira, P.-
dc.contributor.authorLemos, E. Teixeira de-
dc.contributor.authorParada, B.-
dc.contributor.authorBaptista, S.-
dc.contributor.authorFigueiredo, A.-
dc.contributor.authorSantos-Silva, A.-
dc.contributor.authorCosta-Almeida, C.-
dc.contributor.authorMota, A.-
dc.contributor.authorTeixeira, F.-
dc.date.accessioned2008-09-01T14:13:03Z-
dc.date.available2008-09-01T14:13:03Z-
dc.date.issued2007en_US
dc.identifier.citationTransplantation Proceedings. 39:8 (2007) 2494-2500en_US
dc.identifier.urihttps://hdl.handle.net/10316/4705-
dc.description.abstractThe aim of this study was to evaluate the effect of cyclosporine (CsA) on oxidative stress as well as the use of a nitric oxide (NO) donor, the organic nitrate isosorbide-5-mononitrate (Is-5-Mn), to prevent or reverse CsA-induced toxicity, namely on the vascular NO-cGMP pathway or on oxidative equilibrium. The following rat groups (n = 8) were tested: (1) a control group; (2) the CsA group (5 mg/kg/d for 7 weeks); (3) the Is-5-Mn group (150 mg/kg/d, twice a day for 7 weeks); (4) the preventive group (Is-5-Mn + CsA) treated for 2 weeks with Is-5-Mn only, and thereafter with both drugs for 7 weeks; (5) the curative group (CsA + Is-5-Mn) beginning 7 weeks after CsA, and following thereafter with both drugs for 5 weeks. The following parameters were evaluated: aortic cNOS activity and cGMP content; plasma levels of lipid peroxidation (malondialdehyde [MDA] levels); antioxidant capacity (glutathione peroxidase [GPx] and superoxide dismutase [SOD] activities, total antioxidant status, and vitamins A, C, and E); and peroxynitrite formation (3-nitrotyrosine [3-NT] content). Is-5-Mn + CsA therapy showed, when compared with the CsA group, total prevention of CsA-induced NO and cGMP attenuation, and no relevant influence on antioxidant indices, as well as on MDA and 3-NT levels. However, when compared with this CsA group, the curative group (CsA + Is-5-Mn) showed NO-cGMP values only partially reversed, and an enhancement in lipid peroxidation (5.6 ± 1.4 vs 12.78 ± 3.63 [mu]mol/L; P < .05) and in peroxynitrite formation (16.7% incidence of positives vs 83.3% incidence of positives). Our data suggested that nitrate therapy may provide a valid choice to prevent CsA-induced NO-cGMP decrease, without a negative influence on the oxidative equilibrium. However, when the local environment is adverse, as occurs after CsA therapy, Is-5-Mn seemed to enhance the CsA-induced oxidative stress, promoting even worse deleterious effects, probably through the generation of the cytotoxic ROS peroxynitrite.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6VJ0-4PYHTPY-C/1/55829e76e32a26b46c63727f0d20b75cen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleOxidative Stress in Cyclosporine-Induced Hypertension: Evidence of Beneficial Effects or Tolerance Development With Nitrate Therapyen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.transproceed.2007.07.030-
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0003-3401-9554-
crisitem.author.orcid0000-0002-9105-9619-
crisitem.author.orcid0000-0002-2601-0923-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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