Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/4679
DC FieldValueLanguage
dc.contributor.authorDonato, Roberta-
dc.contributor.authorRodrigues, Ricardo J.-
dc.contributor.authorTakahashi, Michiko-
dc.contributor.authorTsai, Ming Chi-
dc.contributor.authorSoto, David-
dc.contributor.authorMiyagi, Kana-
dc.contributor.authorVillafuertes, Rosa Gomez-
dc.contributor.authorCunha, Rodrigo A.-
dc.contributor.authorEdwards, Frances A.-
dc.date.accessioned2008-09-01T14:12:36Z-
dc.date.available2008-09-01T14:12:36Z-
dc.date.issued2008-09-01T14:12:36Z-
dc.identifier.citationCell Calcium. In Press, Corrected Proof:en_US
dc.identifier.urihttps://hdl.handle.net/10316/4679-
dc.description.abstractIn many brain regions, Ca2+ influx through presynaptic P2X receptors influences GABA release from interneurones. In patch-clamp recordings of Purkinje cells (PCs) in rat cerebellar slices, broad spectrum P2 receptor antagonists, PPADS (30 [mu]M) or suramin (12 [mu]M), result in a decreased amplitude and increased failure rate of minimal evoked GABAergic synaptic currents from basket cells. The effect is mimicked by desensitizing P2X1/3-containing receptors with [alpha],[beta]-methylene ATP. This suggests presynaptic facilitation of GABA release via P2XR-mediated Ca2+ influx activated by endogenously released ATP. In contrast, activation of P2Y4 receptors (using UTP, 30 [mu]M, but not P2Y1 or P2Y6 receptor ligands) results in inhibition of GABA release. Immunological studies reveal the presence of most known P2Rs in >=20% of GABAergic terminals in the cerebellum. P2X3 receptors and P2Y4 receptors occur in approximately 60% and 50% of GABAergic synaptosomes respectively and are localized presynaptically. Previous studies report that PC output is also influenced by postsynaptic purinergic receptors located on both PCs and interneurones.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6WCC-4SGTM4N-1/1/18be0bd4d3f723826c492461dcb46423en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPurkinje cellen_US
dc.subjectCerebellar cortexen_US
dc.subjectPurinergicen_US
dc.subjectGABA releaseen_US
dc.subjectCalcium influxen_US
dc.subjectCalcium storesen_US
dc.subjectSynaptic transmissionen_US
dc.subjectPresynaptic receptorsen_US
dc.titleGABA release by basket cells onto Purkinje cells, in rat cerebellar slices, is directly controlled by presynaptic purinergic receptors, modulating Ca2+ influxen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.ceca.2008.03.006-
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-7631-743X-
crisitem.author.orcid0000-0003-2550-6422-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
file0691c57015684e7a892daa4fc3fd7a47.pdf1.04 MBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

25
checked on Nov 11, 2022

WEB OF SCIENCETM
Citations

24
checked on May 2, 2023

Page view(s) 20

692
checked on Mar 26, 2024

Download(s) 20

892
checked on Mar 26, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.