Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/44487
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dc.contributor.authorMarques, M. P. M.-
dc.contributor.authorCarvalho, A. L. M. Batista de-
dc.contributor.authorSakai, V Garcia-
dc.contributor.authorHatter, L-
dc.contributor.authorCarvalho, L. A. E. Batista de-
dc.date.accessioned2017-11-21T11:42:42Z-
dc.date.issued2017-01-25-
dc.identifier.urihttp://hdl.handle.net/10316/44487-
dc.description.abstractThe first neutron scattering study on human nucleated cells is reported, addressing the subject of solvent-slaving to a drug by probing intracellular water upon drug exposure. Inelastic and quasi-elastic neutron scattering spectroscopy with isotope labelling was applied for monitoring interfacial water response to the anticancer drug cisplatin, in the low prognosis human metastatic breast cancer cells MDA-MB-231. Optical vibrational data were also obtained for lyophilised cells. Concentration-dependent dynamical changes evidencing a progressive mobility reduction were unveiled between untreated and cisplatin-exposed samples, concurrent with variations in the native organisation of water molecules within the intracellular medium as a consequence of drug action. The results thus obtained yielded a clear picture of the intracellular water response to cisplatin and constitute the first reported experimental proof of a drug impact on the cytomatrix by neutron techniques. This is an innovative way of tackling a drug's pharmacodynamics, searching for alternative targets of drug action.por
dc.language.isoengpor
dc.rightsembargoedAccess-
dc.titleIntracellular water - an overlooked drug target? Cisplatin impact in cancer cells probed by neutronspor
dc.typearticle-
degois.publication.firstPage2702por
degois.publication.lastPage2713por
degois.publication.issue4por
degois.publication.titlePhysical chemistry chemical physics : PCCPpor
dc.peerreviewedyespor
dc.identifier.doi10.1039/c6cp05198g-
degois.publication.volume19por
dc.date.embargo2018-11-21T11:42:42Z-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitQFM-UC – Molecular Physical-Chemistry R&D Unit-
crisitem.author.researchunitQFM-UC – Molecular Physical-Chemistry R&D Unit-
crisitem.author.orcid0000-0002-8391-0055-
crisitem.author.orcid0000-0002-8059-8537-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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