Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/3866
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dc.contributor.authorFonseca, C. P.-
dc.contributor.authorMontezinho, L. P.-
dc.contributor.authorNabais, C.-
dc.contributor.authorTomé, A. R.-
dc.contributor.authorFreitas, H.-
dc.contributor.authorGeraldes, C. F. G. C.-
dc.contributor.authorCastro, M. M. C. A.-
dc.date.accessioned2008-08-29T15:35:16Z-
dc.date.available2008-08-29T15:35:16Z-
dc.date.issued2004en_US
dc.identifier.citationBiochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1691:2-3 (2004) 79-90en_US
dc.identifier.urihttps://hdl.handle.net/10316/3866-
dc.description.abstractLi+ transport, intracellular immobilisation and Li+/Mg2+ competition were studied in Li+-loaded bovine chromaffin cells. Li+ influx rate constants, ki, obtained by atomic absorption (AA) spectrophotometry, in control (without and with ouabain) and depolarising (without and with nitrendipine) conditions, showed that L-type voltage-sensitive Ca2+ channels have an important role in Li+ uptake under depolarising conditions. The Li+ influx apparent rate constant, kiapp, determined under control conditions by 7Li NMR spectroscopy with the cells immobilised and perfused, was much lower than the AA-determined value for the cells in suspension. Loading of cell suspensions with 15 mmol l-1 LiCl led, within 90 min, to a AA-measured total intracellular Li+ concentration, [Li+]iT=11.39±0.56 mmol (l cells)-1, very close to the steady state value. The intracellular Li+ T1/T2 ratio of 7Li NMR relaxation times of the Li+-loaded cells reflected a high degree of Li+ immobilisation in bovine chromaffin cells, similar to neuroblastoma, but larger than for lymphoblastoma and erythrocyte cells. A 52% increase in the intracellular free Mg2+ concentration, [Delta][Mg2+]f=0.27±0.05 mmol (l cells)-1 was measured for chromaffin cells loaded with the Mg2+-specific fluorescent probe furaptra, after 90-min loading with 15 mmol l-1 LiCl, using fluorescence spectroscopy, indicating significant displacement of Mg2+ by Li+ from its intracellular binding sites. Comparison with other cell types showed that the extent of intracellular Li+/Mg2+ competition at the same Li+ loading level depends on intracellular Li+ transport and immobilisation in a cell-specific manner, being maximal for neuroblastoma cells.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T20-4BF003B-1/1/629d75aadfe4679bd346afea7f719029en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectLi+ affinityen_US
dc.subjectIntracellular Mg2+en_US
dc.subjectFluorescenceen_US
dc.subjectAtomic absorptionen_US
dc.titleEffects of Li+ transport and intracellular binding on Li+/Mg2+ competition in bovine chromaffin cellsen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.bbamcr.2003.12.005-
uc.controloAutoridadeSim-
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCFE - Centre for Functional Ecology - Science for People & the Planet-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0001-8671-989X-
crisitem.author.orcid0000-0002-1907-9615-
crisitem.author.orcid0000-0002-0837-8329-
crisitem.author.orcid0000-0001-6811-3878-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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