Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/3866
DC Field | Value | Language |
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dc.contributor.author | Fonseca, C. P. | - |
dc.contributor.author | Montezinho, L. P. | - |
dc.contributor.author | Nabais, C. | - |
dc.contributor.author | Tomé, A. R. | - |
dc.contributor.author | Freitas, H. | - |
dc.contributor.author | Geraldes, C. F. G. C. | - |
dc.contributor.author | Castro, M. M. C. A. | - |
dc.date.accessioned | 2008-08-29T15:35:16Z | - |
dc.date.available | 2008-08-29T15:35:16Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1691:2-3 (2004) 79-90 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/3866 | - |
dc.description.abstract | Li+ transport, intracellular immobilisation and Li+/Mg2+ competition were studied in Li+-loaded bovine chromaffin cells. Li+ influx rate constants, ki, obtained by atomic absorption (AA) spectrophotometry, in control (without and with ouabain) and depolarising (without and with nitrendipine) conditions, showed that L-type voltage-sensitive Ca2+ channels have an important role in Li+ uptake under depolarising conditions. The Li+ influx apparent rate constant, kiapp, determined under control conditions by 7Li NMR spectroscopy with the cells immobilised and perfused, was much lower than the AA-determined value for the cells in suspension. Loading of cell suspensions with 15 mmol l-1 LiCl led, within 90 min, to a AA-measured total intracellular Li+ concentration, [Li+]iT=11.39±0.56 mmol (l cells)-1, very close to the steady state value. The intracellular Li+ T1/T2 ratio of 7Li NMR relaxation times of the Li+-loaded cells reflected a high degree of Li+ immobilisation in bovine chromaffin cells, similar to neuroblastoma, but larger than for lymphoblastoma and erythrocyte cells. A 52% increase in the intracellular free Mg2+ concentration, [Delta][Mg2+]f=0.27±0.05 mmol (l cells)-1 was measured for chromaffin cells loaded with the Mg2+-specific fluorescent probe furaptra, after 90-min loading with 15 mmol l-1 LiCl, using fluorescence spectroscopy, indicating significant displacement of Mg2+ by Li+ from its intracellular binding sites. Comparison with other cell types showed that the extent of intracellular Li+/Mg2+ competition at the same Li+ loading level depends on intracellular Li+ transport and immobilisation in a cell-specific manner, being maximal for neuroblastoma cells. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T20-4BF003B-1/1/629d75aadfe4679bd346afea7f719029 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Li+ affinity | en_US |
dc.subject | Intracellular Mg2+ | en_US |
dc.subject | Fluorescence | en_US |
dc.subject | Atomic absorption | en_US |
dc.title | Effects of Li+ transport and intracellular binding on Li+/Mg2+ competition in bovine chromaffin cells | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/j.bbamcr.2003.12.005 | - |
uc.controloAutoridade | Sim | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0001-8671-989X | - |
crisitem.author.orcid | 0000-0002-1907-9615 | - |
crisitem.author.orcid | 0000-0002-0837-8329 | - |
crisitem.author.orcid | 0000-0001-6811-3878 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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file810210ba5e6b45778c6c471aa200ff1c.pdf | 323.55 kB | Adobe PDF | View/Open |
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