Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27821
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dc.contributor.authorBaptista, Sofia-
dc.contributor.authorLasgi, Charlène-
dc.contributor.authorBenstaali, Caroline-
dc.contributor.authorMilhazes, Nuno-
dc.contributor.authorBorges, Fernanda-
dc.contributor.authorFontes-Ribeiro, Carlos-
dc.contributor.authorAgasse, Fabienne-
dc.contributor.authorSilva, Ana Paula-
dc.date.accessioned2014-12-09T11:11:01Z-
dc.date.available2014-12-09T11:11:01Z-
dc.date.issued2014-09-
dc.identifier.citationBAPTISTA, Sofia [et. al] - Methamphetamine decreases dentate gyrus stem cell self-renewal and shifts the differentiation towards neuronal fate. "Stem Cell Research". ISSN 1873-5061. Vol. 13 Nº. 2 (2014) p. 329–341por
dc.identifier.issn1873-5061-
dc.identifier.urihttp://hdl.handle.net/10316/27821-
dc.description.abstractMethamphetamine (METH) is a highly addictive psychostimulant drug of abuse that negatively interferes with neurogenesis. In fact, we have previously shown that METH triggers stem/progenitor cell death and decreases neuronal differentiation in the dentate gyrus (DG). Still, little is known regarding its effect on DG stem cell properties. Herein, we investigate the impact of METH on mice DG stem/progenitor cell self-renewal functions. METH (10 nM) decreased DG stem cell self-renewal, while 1 nM delayed cell cycle in the G0/G1-to-S phase transition and increased the number of quiescent cells (G0 phase), which correlated with a decrease in cyclin E, pEGFR and pERK1/2 protein levels. Importantly, both drug concentrations (1 or 10 nM) did not induce cell death. In accordance with the impairment of self-renewal capacity, METH (10 nM) decreased Sox2+/Sox2+ while increased Sox2−/Sox2− pairs of daughter cells. This effect relied on N-methyl-d-aspartate (NMDA) signaling, which was prevented by the NMDA receptor antagonist, MK-801 (10 μM). Moreover, METH (10 nM) increased doublecortin (DCX) protein levels consistent with neuronal differentiation. In conclusion, METH alters DG stem cell properties by delaying cell cycle and decreasing self-renewal capacities, mechanisms that may contribute to DG neurogenesis impairment followed by cognitive deficits verified in METH consumers.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.titleMethamphetamine decreases dentate gyrus stem cell self-renewal and shifts the differentiation towards neuronal fatepor
dc.typearticlepor
degois.publication.firstPage329por
degois.publication.lastPage341por
degois.publication.issue2por
degois.publication.titleStem Cell Researchpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1873506114000920por
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.scr.2014.08.003-
degois.publication.volume13por
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
I&D IBILI - Artigos em Revistas Internacionais
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