Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/27255
DC FieldValueLanguage
dc.contributor.authorMargalho, Cláudia-
dc.contributor.authorGallardo, Eugenia-
dc.contributor.authorCastanheira, Alice-
dc.contributor.authorVieira, Duarte Nuno-
dc.contributor.authorLópez-Rivadulla, Manuel-
dc.contributor.authorReal, Francisco Corte-
dc.date.accessioned2014-10-14T10:03:16Z-
dc.date.available2014-10-14T10:03:16Z-
dc.date.issued2013-08-23-
dc.identifier.citationMARGALHO, Claúdia [et. al] - A validated procedure for detection and quantitation of salvinorin a in pericardial fluid, vitreous humor, whole blood and plasma using solid phase extraction and gas chromatography–mass spectrometry. "Journal of Chromatography A". ISSN 0021-9673. Vol. 1304 (2013) p. 203-210por
dc.identifier.issn0021-9673-
dc.identifier.urihttps://hdl.handle.net/10316/27255-
dc.description.abstractThe use of vitreous humor and pericardial fluid as alternative matrices to blood and plasma in the field of forensic toxicology is described to quantitate low levels of Salvinorin A using ethion as internal standard. The method was optimized and fully validated using international accepted guidelines. The developed methodology utilizes a solid phase extraction procedure coupled to gas chromatography mass spectrometry operated in the selected ion monitoring mode. The method was linear in the range of 5.0–100 ng/mL with determination coefficients higher than 0.99 in 100 μL of vitreous humor and in 250 μL of each matrix pericardial fluid, whole blood and plasma. The limits of detection and quantitation were experimentally determined as 5.0 ng/mL, intra-day precision, intermediate precision and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The sample cleanup step presented mean efficiencies between 80 and 106% in the different biological specimens analyzed. According to the low volumes of samples used, and the low limits achieved using a single quadrupole mass spectrometer, which is available in most laboratories, we can conclude that the validated methodology is sensitive and simple and is suitable for the application in forensic toxicology laboratories for the routine analysis of Salvinorin A in both conventional and unconventional biological samples.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectSalvinorin Apor
dc.subjectBiological specimenspor
dc.subjectSPEpor
dc.subjectGC-MS-EIpor
dc.titleA validated procedure for detection and quantitation of salvinorin a in pericardial fluid, vitreous humor, whole blood and plasma using solid phase extraction and gas chromatography–mass spectrometrypor
dc.typearticlepor
degois.publication.firstPage203por
degois.publication.lastPage210por
degois.publication.titleJournal of Chromatography Apor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0021967313010753por
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.chroma.2013.07.031-
degois.publication.volume1304por
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.orcid0000-0002-7366-6765-
crisitem.author.orcid0000-0003-1495-9362-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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