Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27088
DC FieldValueLanguage
dc.contributor.authorDomingues, N.-
dc.contributor.authorPelletier, J.-
dc.contributor.authorOstenson, C.-G.-
dc.contributor.authorCastro, M. M. C. A.-
dc.date.accessioned2014-09-29T14:03:35Z-
dc.date.available2014-09-29T14:03:35Z-
dc.date.issued2014-02-
dc.identifier.citationDOMINGUES, N. [et al.] - Therapeutic properties of VO(dmpp)2 as assessed by in vitro and in vivo studies in type 2 diabetic GK rats. "Journal of Inorganic Biochemistry". ISSN 0162-0134. Vol. 131 (2014) p. 115-122por
dc.identifier.issn0162-0134-
dc.identifier.urihttp://hdl.handle.net/10316/27088-
dc.description.abstractThe bis(1,2-dimethyl-3-hydroxy-4-pyridinonato)oxidovanadium(IV), VO(dmpp)2, has shown anti-diabetic effects by in vitro studies in Wistar (W) rat adipocytes and in vivo in obese Zucker rats. The aim of this work is to confirm the therapeutic properties of VO(dmpp)2 in non-obese type 2 diabetic Goto-Kakizaki (GK) rats. An in vivo study was carried out, treating W and GK rats during 21 days with a daily dose of VO(dmpp)2 (44 μmol/kg). It was shown that VO(dmpp)2 doesn't affect the normal increase of body weight of both W and GK rats, after 8 days of treatment ameliorates glycemia in GK rats (8.4 ± 0.3 vs 10.1 ± 0.2 mM in GK control, P < 0.001) but doesn't interfere with glucose levels in W rats and, after 21 days of treatment, improves the glucose intolerant profile of GK rats (13.1 ± 0.5 vs 20.6 ± 0.7 mM/min in GK control, P < 0.001), despite no increase of plasma insulin levels during glucose tolerance test. Additionally, it was demonstrated that VO(dmpp)2 significantly enhances [3-3H]-glucose uptake by W and GK rat adipocytes (non-toxic concentration of 100 μM: respectively 193 ± 20 and 254 ± 21%, P < 0.001, relative to the basal value) showing an efficacy similar to insulin 1.72 nM and better than the same concentration of BMOV (P < 0.01). Western blotting revealed that in W and GK rats VO(dmpp)2 significantly promotes IRS2 (P < 0.05) and p-AKT expression (P < 0.001 and P < 0.05, respectively, relative to the respective controls) and in GK animals reduces the increase of PTP1β expression (P < 0.001, relative to GK control).por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectType 2 diabetespor
dc.subjectVO(dmpp)2por
dc.subjectGoto-Kakizaki ratspor
dc.subjectGlucose uptake by adipocytespor
dc.subjectGlucose tolerancepor
dc.subjectInsulin signaling pathwaypor
dc.titleTherapeutic properties of VO(dmpp)2 as assessed by in vitro and in vivo studies in type 2 diabetic GK ratspor
dc.typearticlepor
degois.publication.firstPage115por
degois.publication.lastPage122por
degois.publication.titleJournal of Inorganic Biochemistrypor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0162013413003164por
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.jinorgbio.2013.11.005-
degois.publication.volume131por
uc.controloAutoridadeSim-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextCom Texto completo-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitCoimbra Chemistry Center-
crisitem.author.parentresearchunitFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.orcid0000-0001-6811-3878-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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