Immunonephelometry in cyclosporin therapy

Abstract

Cyclosporin is the most effective drug in human allogenic graf survival. Hypertrichosis, gingival hypertrophy, hypertension, hepatotoxicity have been observed but nephrotocicity seems to be the main problem. Cyclosporine activity seems to be related with peripherical lymphocytes, intraocular levels in systemic administration are not effective, and topical treatment appears to be only useful in corneal disease. Cyclosporine blood levels are important to evaluate intestinal absortion, as well as nephrotoxicity is related with blood levels. Aqueous protein levels are related with inflammatory events in uveitis, and can be easily measured in diary clinic by computorized nephelometry; albumin can be a good reference of blood aqueous barrier “in vivo” with alpha antitripsin directly reflecting inflammatory activity. Evaluation of cyclosporin therapy can be done with only one aqueous tap of 50-100ul and measuring 5 proteins at least.