Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/13030
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Vale, M. G. P. | - |
dc.contributor.author | Carvalho, A. P. | - |
dc.date.accessioned | 2010-04-20T12:30:20Z | - |
dc.date.available | 2010-04-20T12:30:20Z | - |
dc.date.issued | 1980-02-15 | - |
dc.identifier.citation | Biochemical Journal. 186:2 (1980) 461-467 | en_US |
dc.identifier.issn | 0306-3283 | - |
dc.identifier.uri | https://hdl.handle.net/10316/13030 | - |
dc.description | Biochemical Journal. 186:2 (1980) 461-467 | en_US |
dc.description.abstract | Sarcoplasmic-reticulum vesicles were actively loaded with Ca2+ in the presence of phosphate, and the ADP-induced Ca2+ efflux and ATP synthesis were measured as a function of temperature. Arrhenius plots show break points for both processes at about 18 and 37 degrees C. Between 18 and 37 degrees C, Ca2+ efflux and ATP synthesis occur with an activation energy of 67.2-71.4 kJ/mol, whereas it is about 189-210 kJ/mol for temperatures below 18 degrees C. Above 37 degrees C, the rates of ADP-induced Ca2+ release and of ATP synthesis sharply decline until the temperature reaches about 42 degrees C. Above this temperature, the Ca2+ efflux increases again even in absence of ADP, although the synthesis of ATP is inhibited, which reflects leakiness of the vesicles. The results show that the transition temperatures for ADP-induced Ca2+ efflux and for ATP synthesis resemble those for active Ca2+ uptake, which indicates that the same coupling mechanism is involved during the inward and outward Ca2+ translocations across the membrane | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Portland Press | en_US |
dc.rights | openAccess | en_US |
dc.title | Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum | en_US |
dc.type | article | en_US |
degois.publication.firstPage | 461 | en_US |
degois.publication.lastPage | 467 | en_US |
degois.publication.issue | 186:2 | en_US |
degois.publication.title | Biochemical Journal | en_US |
dc.identifier.doi | 10.1042/bj1860461 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Effect of temperature on the reversal.pdf | 946 kB | Adobe PDF | View/Open |
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