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https://hdl.handle.net/10316/12774
Título: | 7-ketocholesterol stimulates differentiation of lens epithelial cells | Autor: | Girão, Henrique Shang, Fu Pereira, Paulo |
Data: | 6-Out-2003 | Editora: | Molecular Vision | Citação: | Molecular Vision. 9 (2003) 497-501 | Resumo: | PURPOSE: To establish if oxysterols stimulate differentiation of lens epithelial cells (LEC). METHODS: Primary cultures of lens epithelial cells were incubated with 7-ketocholesterol (7-keto), 25-hydroxycholesterol (25-OH) or cholesterol at 10 microg/ml for 10 days. Cells incubated with 100 ng/ml basic fibroblast growth factor (b-FGF) were used as positive controls for differentiation. The expression of the differentiation marker p57KIP2, proliferation marker PCNA (Proliferating Cell Nuclear Antigen) and fibers specific proteins gamma-crystallin, CP49, MIP26 following treatment with oxysterols was determined by western blot. Differentiation into fiber cells was further confirmed by counting the number of lentoid bodies formed following incubation with 7-keto. RESULTS: LEC incubated with 7-keto presented higher levels of p57KIP2 and showed expression of fiber specific proteins such as MIP26 and CP49, compared to cells incubated with 25-OH or cholesterol. The differentiation marker p57KIP2 increased over time for cells incubated with 7-keto while there was a decline on the amount of the proliferation marker PCNA. The expression of the fiber specific proteins gamma-crystallin, MIP26 and CP49 was detected after 5 days of incubation with 7-keto. Differentiation was accompanied by a seven-fold increase in the number of lentoid bodies formed. CONCLUSIONS: Results show for the first time that 7-keto inhibits proliferation and stimulates differentiation of lens epithelial cells into fiber cells. The presence of 7-keto in the lens may disrupt the highly regulated differentiation program of LEC, compromising normal lens growth and transparency | URI: | https://hdl.handle.net/10316/12774 | ISSN: | 1090-0535 | Direitos: | openAccess |
Aparece nas coleções: | FMUC Medicina - Artigos em Revistas Internacionais |
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7-ketocholesterol stimulates differentiation of lens epithelial cells.pdf | 171.38 kB | Adobe PDF | Ver/Abrir |
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