Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/113346
Title: Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav
Authors: Neves, Mariana 
Correia, Sandra 
Canhoto, Jorge 
Keywords: ACC; aminoethoxyvinylglycine; ethylene modulation; ethephon; in vitro culture; organogenesis; silver nitrate; tree tomato
Issue Date: 30-Apr-2023
Publisher: MDPI
Project: PTDC/BAAAGR/ 32265/2017 
info:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH/BD/123702/2016/PT 
UIDB/04539/2020 
UIDP/04539/2020 
2020.05137.BD 
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB/04004/2020 
Serial title, monograph or event: Plants
Volume: 12
Issue: 9
Abstract: In de novo shoot organogenesis (DNSO) plant cells develop into new shoots, without the need of an existing meristem. Generally, this process is triggered by wounding and specific growth regulators, such as auxins and cytokinins. Despite the potential significance of the plant hormone ethylene in DNSO, its effect in regeneration processes of woody species has not been thoroughly investigated. To address this gap, Solanum betaceum Cav. was used as an experimental model to explore the role of this hormone on DNSO and potentially extend the findings to other woody species. In this work it was shown that ethylene positively regulates DNSO from tamarillo leaf explants. Ethylene precursors ACC and ethephon stimulated shoot regeneration by increasing the number of buds and shoots regenerated. In contrast, the inhibition of ethylene biosynthesis or perception by AVG and AgNO3 decreased shoot regeneration. Organogenic callus induced in the presence of ethylene precursors showed an upregulated expression of the auxin efflux carrier gene PIN1, suggesting that ethylene may enhance shoot regeneration by affecting auxin distribution prior to shoot development. Additionally, it was found that the de novo shoot meristems induced in explants in which ethylene biosynthesis and perception was suppressed were unable to further develop into elongated shoots. Overall, these results imply that altering ethylene levels and perception could enhance shoot regeneration efficiency in tamarillo. Moreover, we offer insights into the possible molecular mechanisms involved in ethylene-induced shoot regeneration.
URI: https://hdl.handle.net/10316/113346
ISSN: 2223-7747
DOI: 10.3390/plants12091854
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CFE - Artigos em Revistas Internacionais

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