Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/11255
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dc.contributor.authorMorán, M. Carmen-
dc.contributor.authorPais, Alberto A. C. C.-
dc.contributor.authorRamalho, Amilcar-
dc.contributor.authorMiguel, M. Graça-
dc.contributor.authorLindman, Björn-
dc.date.accessioned2009-08-31T07:59:15Z-
dc.date.available2009-08-31T07:59:15Z-
dc.date.issued2009-09-01-
dc.identifier.citationLangmuir. 25:17 (2009) 10263-10270en_US
dc.identifier.issn0743-7463-
dc.identifier.urihttp://hdl.handle.net/10316/11255-
dc.description.abstractAqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited in forming DNA gel particles by interfacial diffusion. We report here the formation of DNA gel particles by mixing solutions of double-stranded DNA with aqueous solutions containing two cationic proteins, lysozyme and protamine sulfate. The effect of the lysozyme/protamine ratio on the degree of DNA entrapment, surface morphology, swelling−deswelling behavior, and kinetics of DNA release has been investigated. By mixing the two proteins, we obtain particles that display higher loading efficiency and loading capacity values, in comparison to those obtained in single-protein systems. Examination of the release profiles has shown that in mixed protein particles, complex, dual-stage release kinetics is obtained. The overall release profile is dependent on the lysozyme/protamine ratio. The obtained profiles, or segments of them, are accuratelly fitted using the zero-order and first-order models, and the Weibull function. Fluorescence microscopy studies have suggested that the formation of these particles is associated with the conservation of the secondary structure of DNA. This study presents a new platform for controlled release of DNA from DNA gel particles formed by interfacial diffusion.en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsopenAccesseng
dc.titleMixed Protein Carriers for Modulating DNA Releaseen_US
dc.typearticleen_US
dc.identifier.doi10.1021/la901071v-
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-6725-6460-
crisitem.author.orcid0000-0001-7004-2212-
crisitem.author.orcid0000-0002-1601-5501-
crisitem.author.orcid0000-0002-7493-9112-
Appears in Collections:FCTUC Eng.Mecânica - Artigos em Revistas Internacionais
FCTUC Química - Artigos em Revistas Internacionais
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