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https://hdl.handle.net/10316/108850
Title: | Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities | Authors: | Rodrigues, Fernanda Danon, Leon Morales-Aza, Begonia Sikora, Paulina Thors, Valtyr Ferreira, Muriel Gould, Katherine Hinds, Jason Finn, Adam |
Issue Date: | 2016 | Publisher: | Public Library of Science | Project: | investigator-led project grant from Pfizer National Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Evaluation of Interventions at University of Bristol NIH (Pepfar supplement) and Canadian Institute of Health Research. |
Serial title, monograph or event: | PLoS ONE | Volume: | 11 | Issue: | 9 | Abstract: | Prevalence of pneumococcal serotypes in carriage and disease has been described but absolute serotype colonisation densities have not been reported. 515 paediatric nasal swab DNA extracts were subjected to lytA qPCR and molecular serotyping by microarray. Absolute serotype densities were derived from total pneumococcal density (qPCR cycle threshold and standard curve) and relative abundance (microarray) and varied widely. Compared to all serotype densities observed, the strongest evidence of differences was seen for serotypes 21 and 35B (higher) and 3, 38 and non-typeables (lower) (p<0.05) with a similar hierarchy when only a single serotype carriage was assessed. There was no evidence of any overall density differences between children with single or multiple serotypes detected but serotypes with mid-range densities were more prevalent. The hierarchy of distinct pneumococcal serotype carriage densities described here for the first time, may help explain the dynamics of transmission between children. | URI: | https://hdl.handle.net/10316/108850 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0163435 | Rights: | openAccess |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
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Pneumococcal-serotypes-colonise-the-nasopharynx-in-children-at-different-densitiesPLoS-ONE.pdf | 1.42 MB | Adobe PDF | View/Open |
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