Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106535
DC Field | Value | Language |
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dc.contributor.author | Souto, Eliana B. | - |
dc.contributor.author | Souto, Selma B. | - |
dc.contributor.author | Zielińska, Aleksandra | - |
dc.contributor.author | Durazzo, Alessandra | - |
dc.contributor.author | Lucarini, Massimo | - |
dc.contributor.author | Santini, Antonello | - |
dc.contributor.author | Horbańczuk, Olaf K. | - |
dc.contributor.author | Atanasov, Atanas G. | - |
dc.contributor.author | Marques, Conrado | - |
dc.contributor.author | Andrade, Luciana N. | - |
dc.contributor.author | Silva, Amélia M. | - |
dc.contributor.author | Severino, Patrícia | - |
dc.date.accessioned | 2023-04-06T11:32:14Z | - |
dc.date.available | 2023-04-06T11:32:14Z | - |
dc.date.issued | 2020-02-16 | - |
dc.identifier.issn | 1999-4923 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106535 | - |
dc.description.abstract | We have developed a new cationic solid lipid nanoparticle (SLN) formulation, composed of Compritol ATO 888, poloxamer 188 and cetyltrimethylammonium bromide (CTAB), to load perillaldehyde 1,2-epoxide, and surface-tailored with a monoclonal antibody for site-specific targeting of human epithelial growth receptor 2 (HER2). Perillaldehyde 1,2-epoxide-loaded cationic SLN (cPa-SLN), with a mean particle size (z-Ave) of 275.31 ± 4.78 nm and polydispersity index (PI) of 0.303 ± 0.081, were produced by high shear homogenization. An encapsulation efficiency of cPa-SLN above 80% was achieved. The release of perillaldehyde 1,2-epoxide from cationic SLN followed the Korsemeyer-Peppas kinetic model, which is typically seen in nanoparticle formulations. The lipid peroxidation of cPa-SLN was assessed by the capacity to produce thiobarbituric acid-reactive substances, while the antioxidant activity was determined by the capacity to scavenge the stable radical DPPH. The surface functionalization of cPa-SLN with the antibody was done via streptavidin-biotin interaction, monitoring z-Ave, PI and ZP of the obtained assembly (cPa-SLN-SAb), as well as its stability in phosphate buffer. The effect of plain cationic SLN (c-SLN, monoterpene free), cPa-SLN and cPa-SLN-SAb onto the MCF-7 cell lines was evaluated in a concentration range from 0.01 to 0.1 mg/mL, confirming that streptavidin adsorption onto cPa-SLN-SAb improved the cell viability in comparison to the cationic cPa-SLN. | pt |
dc.description.sponsorship | The authors wish to acknowledge the financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), and from FAPITEC/SE (Fundação de Apoio à Pesquisa e Inovação Tecnológica do Estado de Sergipe). E.B. Souto acknowledges the sponsorship of the projects M-ERA-NET-0004/2015-PAIRED and UIDB/04469/2020, receiving support from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, and co-financed by FEDER, under the Partnership Agreement PT2020. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | UIDB/04469/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | perillaldehyde 1,2-epoxide | pt |
dc.subject | Compritol ATO 888 | pt |
dc.subject | cationic SLN | pt |
dc.subject | streptavidin adsorption | pt |
dc.subject | MCF-7 cells | pt |
dc.title | Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines | pt |
dc.type | article | - |
degois.publication.firstPage | 161 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | Pharmaceutics | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/pharmaceutics12020161 | pt |
degois.publication.volume | 12 | pt |
dc.date.embargo | 2020-02-16 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Centre of Biological Engineering of the University of Minho | - |
crisitem.author.orcid | 0000-0002-9737-6017 | - |
crisitem.author.orcid | 0000-0003-2603-1377 | - |
crisitem.author.orcid | 0000-0001-6527-6612 | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Perillaldehyde-12epoxide-loaded-SLNtailored-mAb-Production-physicochemical-characterization-and-in-vitro-cytotoxicity-profile-in-MCF7-cell-linesPharmaceutics.pdf | 1.28 MB | Adobe PDF | View/Open |
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