Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106532
DC Field | Value | Language |
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dc.contributor.author | Pereira, Patrícia | - |
dc.contributor.author | Barreira, Maria | - |
dc.contributor.author | Cruz, Carla | - |
dc.contributor.author | Tomás, Joana | - |
dc.contributor.author | Luís, Ângelo | - |
dc.contributor.author | Pedro, Augusto Q. | - |
dc.contributor.author | Queiroz, João A. | - |
dc.contributor.author | Sousa, Fani | - |
dc.date.accessioned | 2023-04-06T11:12:07Z | - |
dc.date.available | 2023-04-06T11:12:07Z | - |
dc.date.issued | 2020-10-15 | - |
dc.identifier.issn | 1424-8247 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106532 | - |
dc.description.abstract | The efficacy of brain therapeutics is largely hampered by the presence of the blood-brain barrier (BBB), mainly due to the failure of most (bio) pharmaceuticals to cross it. Accordingly, this study aims to develop nanocarriers for targeted delivery of recombinant precursor microRNA (pre-miR-29b), foreseeing a decrease in the expression of the BACE1 protein, with potential implications in Alzheimer's disease (AD) treatment. Stearic acid (SA) and lactoferrin (Lf) were successfully exploited as brain-targeting ligands to modify cationic polymers (chitosan (CS) or polyethyleneimine (PEI)), and its BBB penetration behavior was evaluated. The intracellular uptake of the dual-targeting drug delivery systems by neuronal cell models, as well as the gene silencing efficiency of recombinant pre-miR-29b, was analyzed in vitro. Labeled pre-miR-29b-CS/PEI-SA-Lf systems showed very strong fluorescence in the cytoplasm and nucleus of RBE4 cells, being verified the delivery of pre-miR-29b to neuronal cells after 1 h transfection. The experiment of transport across the BBB showed that CS-SA-Lf delivered 65% of recombinant pre-miR-29b in a period of 4 h, a significantly higher transport ratio than the 42% found for PEI-SA-Lf in the same time frame. Overall, a novel procedure for the dual targeting of DDS is disclosed, opening new perspectives in nanomedicines delivery, whereby a novel drug delivery system harvests the merits and properties of the different immobilized ligands. | pt |
dc.description.sponsorship | This work was partially supported by the Portuguese Foundation for Science and Technology (FCT), through the project Pest-OE/SAU/UI0709/2014, UIDB/50011/2020 and UIDP/50011/2020, UIDB/00285/2020, and by the project PTDC/BII-BBF/29496/2017 (PUREmiRSILs) funded by FEDER, through the COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI), and by national funds (OE), through FCT/MCTES. The authors also acknowledge the PPBI-Portuguese Platform of BioImaging through the project POCI-01-0145-FEDER-022122 and Rede Nacional de RMN (PTNMR), supported by FCT-MCTES (ROTEIRO/0031/2013—PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL, and FCT through PIDDAC. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | Pest-OE/SAU/UI0709/2014 | pt |
dc.relation | UIDB/50011/2020 | pt |
dc.relation | UIDP/50011/2020 | pt |
dc.relation | UIDB/50011/2020 | pt |
dc.relation | UIDP/50011/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC/BII-BBF/29496/2017/PT/Ionic Liquid-based supports for pre-miRNAs purification targeting Alzheimer's disease | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA/22161/2016/PT/Portuguese Nuclear Magnetic Resonance Network | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | blood–brain barrier | pt |
dc.subject | chitosan | pt |
dc.subject | drug delivery system | pt |
dc.subject | lactoferrin | pt |
dc.subject | polyethyleneimine | pt |
dc.subject | recombinant miRNA | pt |
dc.title | Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes | pt |
dc.type | article | - |
degois.publication.firstPage | 314 | pt |
degois.publication.issue | 10 | pt |
degois.publication.title | Pharmaceuticals | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ph13100314 | pt |
degois.publication.volume | 13 | pt |
dc.date.embargo | 2020-10-15 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.author.orcid | 0000-0002-5665-5271 | - |
Appears in Collections: | I&D CEMMPRE - Artigos em Revistas Internacionais FCTUC Eng.Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Braintargeted-delivery-of-premir29b-using-lactoferrinstearic-acidmodifiedchitosan-polyethyleneimine-polyplexesPharmaceuticals.pdf | 3.52 MB | Adobe PDF | View/Open |
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