Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/10551
DC FieldValueLanguage
dc.contributor.authorPinto, Maria F. V.-
dc.contributor.authorMorán, M. Carmen-
dc.contributor.authorMiguel, M. Graça-
dc.contributor.authorLindman, Björn-
dc.contributor.authorJurado, Amália S.-
dc.contributor.authorPais, Alberto A. C. C.-
dc.date.accessioned2009-07-08T08:41:23Z-
dc.date.available2009-07-08T08:41:23Z-
dc.date.issued2009-06-08-
dc.identifier.citationBiomacromolecules. 10:6 (2009) 1319-1323en_US
dc.identifier.issn1525-7797-
dc.identifier.urihttp://hdl.handle.net/10316/10551-
dc.description.abstractThis work addresses the influence of solution inhomogeneity on conformation, aggregation, and coil/globule and bundle/single chain coexistence of T4 DNA molecules. The inhomogeneity is induced by mixing two solutions containing, respectively, protamine and DNA, with different relative concentrations, but aiming at producing the same final concentrations. The study was conducted by means of fluorescence microscopy (FM), complemented with scanning electron microscopy (SEM). It is shown that the degree of precipitation, the structures formed, and the relative population of compacted and unfolded structures are highly dependent on the method of preparation of the mixtures that contain the DNA/protamine complexes. Most of the structures reported in the literature, that is, overcharged/undercharged globules, toroids, chains internally segregated, and bundles composed of several chains were observed in our different mixtures of fixed final concentration.en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsopenAccesseng
dc.titleControlling the Morphology in DNA Condensation and Precipitationen_US
dc.typearticleen_US
dc.identifier.doi10.1021/bm900211j-
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-1601-5501-
crisitem.author.orcid0000-0002-7493-9112-
crisitem.author.orcid0000-0001-7095-5337-
crisitem.author.orcid0000-0002-6725-6460-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
FCTUC Química - Artigos em Revistas Internacionais
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