Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/105248
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mouritzen, Michelle V. | - |
dc.contributor.author | Petkovic, Marija | - |
dc.contributor.author | Qvist, Katrine | - |
dc.contributor.author | Poulsen, Steen S. | - |
dc.contributor.author | Alarico, Susana | - |
dc.contributor.author | Leal, Ermelindo C. | - |
dc.contributor.author | Dalgaard, Louise T. | - |
dc.contributor.author | Empadinhas, Nuno | - |
dc.contributor.author | Carvalho, Eugenia | - |
dc.contributor.author | Jenssen, Håvard | - |
dc.date.accessioned | 2023-02-10T12:31:53Z | - |
dc.date.available | 2023-02-10T12:31:53Z | - |
dc.date.issued | 2021-03-12 | - |
dc.identifier.issn | 2329-0501 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/105248 | - |
dc.description.abstract | Bovine lactoferricin (LFcinB) has antimicrobial and immunomodulatory properties; however, the effects on diabetic wound healing remain poorly understood. The wound healing potential of LFcinB was investigated with in vitro, ex vivo, and in vivo models. Cell migration and proliferation were tested on keratinocytes and on porcine ears. A type 1 diabetic mouse model was also used to evaluate wound healing kinetics, bacterial diversity patterns, and the effect of LFcinB on oxidative stress, macrophage phenotype, angiogenesis, and collagen deposition. LFcinB increased keratinocyte migration in vitro (p < 0.05) and ex vivo (p < 0.001) and improved wound healing in diabetic mice (p < 0.05), though not in normoglycemic control mice. In diabetic mouse wounds, LFcinB treatment led to the eradication of Bacillus pumilus, a decrease in Staphylococcus aureus, and an increase in the Staphylococcus xylosus prevalence. LFcinB increased angiogenesis in diabetic mice (p < 0.01), but this was decreased in control mice (p < 0.05). LFcinB improved collagen deposition in both diabetic and control mice (p < 0.05). Both oxidative stress and the M1-to-M2 macrophage ratios were decreased in LFcinB-treated wounds of diabetic animals (p < 0.001 and p < 0.05, respectively) compared with saline, suggesting a downregulation of inflammation in diabetic wounds. In conclusion, LFcinB treatment demonstrated noticeable positive effects on diabetic wound healing. | pt |
dc.language.iso | eng | pt |
dc.publisher | Elsevier | pt |
dc.relation | Danish Council for Independent Research, Technology and Production (grant 4005-00029). | pt |
dc.relation | Lundbeck R275-2017- 2793 and Erasmus+ F2: 2015-5577 | pt |
dc.relation | UID/NEU/04539/2013 | pt |
dc.relation | POCI- 01-0145-FEDER-007440 | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | Healthy Aging 2020- CENTRO-01-0145-FEDER-000012-N2323 | pt |
dc.relation | DL57/2016/CP1448/ CT0024 | pt |
dc.relation | NIGMS P20GM109096 | pt |
dc.relation | 5P30-AG028718 | pt |
dc.relation | EFSD European Research Programme in Microvascular Complications/Novartis Pharma AG | pt |
dc.relation | Infarmed grant FIS-FIS- 2015-01_DIA_20150630-144 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt |
dc.subject | bacterial diversity; bovine lactoferricin; collagen deposition; diabetes; immunomodulation; inflammatory cytokines; macrophage polarization; wound healing | pt |
dc.title | Improved diabetic wound healing by LFcinB is associated with relevant changes in the skin immune response and microbiota | pt |
dc.type | article | - |
degois.publication.firstPage | 726 | pt |
degois.publication.lastPage | 739 | pt |
degois.publication.title | Molecular Therapy - Methods and Clinical Development | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1016/j.omtm.2021.02.008 | pt |
degois.publication.volume | 20 | pt |
dc.date.embargo | 2021-03-12 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-1615-6099 | - |
crisitem.author.orcid | 0000-0003-1748-9861 | - |
crisitem.author.orcid | 0000-0001-8938-7560 | - |
crisitem.author.orcid | 0000-0001-6264-3632 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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1-s2.0-S2329050121000231-main.pdf | 2.36 MB | Adobe PDF | View/Open |
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