Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/10328
DC FieldValueLanguage
dc.contributor.authorDias, Rita S.-
dc.contributor.authorInnerlohinger, Josef-
dc.contributor.authorGlatter, Otto-
dc.contributor.authorMiguel, Maria G.-
dc.contributor.authorLindman, Björn-
dc.date.accessioned2009-06-24T16:02:26Z-
dc.date.available2009-06-24T16:02:26Z-
dc.date.issued2005-05-26-
dc.identifier.citationThe Journal of Physical Chemistry B. 109:20 (2005) 10458-10463en_US
dc.identifier.issn1520-6106-
dc.identifier.urihttp://hdl.handle.net/10316/10328-
dc.description.abstractThe compaction and aggregation of DNA induced by cationic surfactants was studied by dynamic light scattering (DLS). Furthermore, the effect on surfactant-compacted DNA of the addition of nonionic amphiphiles and salt was studied. When using sufficiently low amounts of DNA and cetyltrimethylammonium bromide (CTAB), compacted DNA molecules could be monitored by the appearance of a band characterized by lower hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. Notably, we observed a region where compacted molecules coexist with extended ones; these two populations were found to be stable with time. For higher concentrations of CTAB, only compacted molecules were observed and the size of the particles increased with time indicating aggregation. The number of globules present in the coexistence region increased linearly with the surfactant concentrations, as given by the area of the band corresponding to this population, which indicates a double-cooperativity of the binding. The DLS experiments were in good agreement with previous fluorescence microscopy studies, with certain advantages over this technique since there is no need to add fluorescence dyes and antioxidants. Furthermore, it allows the study of molecules which are too small to be visualized by fluorescence microscopy.en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsopenAccesseng
dc.titleCoil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Studyen_US
dc.typearticleen_US
dc.identifier.doi10.1021/jp0444464-
uc.controloAutoridadeSim-
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-1601-5501-
crisitem.author.orcid0000-0002-7493-9112-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
Coil-Globule Transition of DNA Molecules Induced.pdf107.13 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

107
checked on May 29, 2020

WEB OF SCIENCETM
Citations 1

106
checked on Nov 2, 2021

Page view(s)

227
checked on Nov 24, 2021

Download(s)

227
checked on Nov 24, 2021

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.